Quantitative analysis of RNA-protein interactions on a massively parallel array reveals biophysical and evolutionary landscapes
نویسندگان
چکیده
منابع مشابه
Quantitative analysis of RNA-protein interactions on a massively parallel array for mapping biophysical and evolutionary landscapes
RNA-protein interactions drive fundamental biological processes and are targets for molecular engineering, yet quantitative and comprehensive understanding of the sequence determinants of affinity remains limited. Here we repurpose a high-throughput sequencing instrument to quantitatively measure binding and dissociation of MS2 coat protein to >107 RNA targets generated on a flow-cell surface b...
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15 صفحه اولQuantitative proteomic analysis reveals concurrent RNA-protein interactions and identifies new RNA-binding proteins in Saccharomyces cerevisiae.
A growing body of evidence supports the existence of an extensive network of RNA-binding proteins (RBPs) whose combinatorial binding affects the post-transcriptional fate of every mRNA in the cell-yet we still do not have a complete understanding of which proteins bind to mRNA, which of these bind concurrently, and when and where in the cell they bind. We describe here a method to identify the ...
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ژورنال
عنوان ژورنال: Nature Biotechnology
سال: 2014
ISSN: 1087-0156,1546-1696
DOI: 10.1038/nbt.2880